Journal: Toxicology and applied pharmacology

Article Title: Cardiovascular alterations at different stages of hypertension development during ethanol consumption: time-course of vascular and autonomic changes.

doi: 10.1016/j.taap.2014.08.012

Figure Lengend Snippet: Fig. 2. (Left) Changes in mean arterial pressure (ΔMAP) evoked by increasing concentrations of the selective α1-adrenoceptor agonist phenylephrine in animals receiving water (control groups) or increasing concentration of ethanol in drinking water (first week: 5%, second week: 10%, third week: 20%, and fourth week 20% v/v). Circles represent the mean ± SEM. (Right) Maximal effect (Emax) and dose at 50% of the MAP range (ED50) for phenylephrine dose–response curves. The bars represent the mean ± SEM. *P b 0.05 vs respective control group, two- way ANOVA followed by Bonferroni's post hoc test.

Article Snippet: The membranes were blocked by incubation with blocking buffer (10%BSA in TBSt–1.6MNaCl and 0.2MTris, 0.1%Tween 20) for 2 h. After blocking, blots were incubated overnight at 4 °C with primary antibodies to α1a-adrenoceptor (1:1000 dilution; c-19, sc1477, Santa Cruz Biotechnology, Santa Cruz, CA, USA), β2-adrenoceptor (1:500 dilution; M-20, sc-570, Santa Cruz Biotechnology), and β-actin (1:10,000 dilution; 13E5, Rabbit mAB #4970, Cell Signaling Technology).

Techniques: Control, Concentration Assay

Journal: Toxicology and applied pharmacology

Article Title: Cardiovascular alterations at different stages of hypertension development during ethanol consumption: time-course of vascular and autonomic changes.

doi: 10.1016/j.taap.2014.08.012

Figure Lengend Snippet: Fig. 4. Time-course of relative protein levels (protein level/β-actin) of α1a- and β2-adrenoceptor in the mesenteric arterial bed (left) and aorta (right) in animals receiving water (control groups) or increasing concentration of ethanol in drinking water (first week: 5%, second week: 10%, third week: 20%, and fourth week 20% v/v). Circles represent the mean ± SEM. *P b 0.05 vs respective control group, two-way ANOVA followed by Bonferroni's post hoc test.

Article Snippet: The membranes were blocked by incubation with blocking buffer (10%BSA in TBSt–1.6MNaCl and 0.2MTris, 0.1%Tween 20) for 2 h. After blocking, blots were incubated overnight at 4 °C with primary antibodies to α1a-adrenoceptor (1:1000 dilution; c-19, sc1477, Santa Cruz Biotechnology, Santa Cruz, CA, USA), β2-adrenoceptor (1:500 dilution; M-20, sc-570, Santa Cruz Biotechnology), and β-actin (1:10,000 dilution; 13E5, Rabbit mAB #4970, Cell Signaling Technology).

Techniques: Control, Concentration Assay